Related Experiment Video

Updated: Aug 1, 2025

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
08:52

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Published on: March 18, 2022

3.0K

Modeling Osteoarthritis: MiR-16-5p Attenuates IL-1β Induced Chondrocyte Dysfunction by Targeting MAP2K1 through the

Ping Xu1, Xuelong Zhang2, Qian Li3

  • 1Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, Hubei, China.

Annals of Clinical and Laboratory Science
|April 24, 2023
PubMed
Abstract

Insights

MicroRNA-16-5p protects chondrocytes from osteoarthritis damage by targeting MAP2K1 and inhibiting the MAPK pathway. This finding offers a potential therapeutic strategy for osteoarthritis, a debilitating joint disease.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Osteoarthritis (OA) is a degenerative joint disease causing significant morbidity.
  • MAP2K1 has been identified as a potential therapeutic target in OA, but its precise role and mechanism are unclear.

Purpose of the Study:

  • To investigate the biological significance of MAP2K1 in OA.
  • To elucidate the regulatory mechanism of MAP2K1 in OA pathogenesis.

Main Methods:

  • Human chondrocyte cell line (CHON-001) stimulated with Interleukin-1β (IL-1β) to model OA in vitro.
  • Assessed cell viability, apoptosis, protein levels, and gene expression.
  • Confirmed the interaction between miR-16-5p and MAP2K1 using a luciferase reporter assay.

Main Results:

  • IL-1β induced chondrocyte injury, decreased viability, and increased apoptosis.
  • IL-1β upregulated MAP2K1 expression; MAP2K1 depletion protected against IL-1β-induced injury.
  • miR-16-5p directly targeted MAP2K1, and its upregulation counteracted MAP2K1's effects.
  • miR-16-5p suppressed IL-1β-induced MAPK pathway activation.

Conclusions:

  • MiR-16-5p mitigates IL-1β-induced chondrocyte damage by targeting MAP2K1.
  • This interaction inactivates the MAPK signaling pathway, offering a novel therapeutic avenue for OA.
Keywords:
MAP2K1MAPK signalingOsteoarthritischondrocyte dysfunctionmiR-16-5p

More Related Videos

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.9K
A Reproducible Cartilage Impact Model to Generate Post-Traumatic Osteoarthritis in the Rabbit08:42

A Reproducible Cartilage Impact Model to Generate Post-Traumatic Osteoarthritis in the Rabbit

Published on: November 21, 2023

1.2K

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.7K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.5K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.0K