[Competitive interrelations between bilirubin and x-ray contrast agents in binding with human serum albumin]

It was shown that bilirubin can be a fluorescent probe for human serum albumin. Parameters of the interaction of bilirubin with albumin were determined. It was found that bilimin and iopanoate decrease affinity of bilirubin to strongly and poorly binding areas of human serum albumin, biliognost and endobil predominantly to its poorly binding sites, and iodamide and verografin exert no influence on binding of bilirubin with albumin. It is concluded that in the clinical practice it is necessary to take into consideration the possibility of an increase in the blood level of free bilirubin in patients during cholecystography with the use of contrast media.