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Updated: Aug 1, 2025
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation
Published on: January 10, 2025
Protective effect of Guanxin Danshen formula on myocardial ischemiareperfusion injury in rats
Lanfang Li1, Bo Liu1, Min Wang1
1Institute of Medicinal Plant Development - Peking Union Medical College and Chinese Academy of Medical Sciences - Beijing, China.
Purpose:
Myocardial ischemia/reperfusion injury (MIRI) leads to myocardial tissue necrosis, which will increase the size of myocardial infarction. The study examined the protective effect and mechanism of the Guanxin Danshen formula (GXDSF) on MIRI in rats.
Methods:
MIRI model was performed in rats; rat H9C2 cardiomyocytes were hypoxia-reoxygenated to establish a cell injury model.
Results:
The GXDSF significantly reduced myocardial ischemia area, reduced myocardial structural injury, decreased the levels of interleukin (IL-1β, IL-6) in serum, decreased the activity of myocardial enzymes, increased the activity of superoxide dismutase (SOD), and reduced glutathione in rats with MIRI. The GXDSF can reduce the expression of nucleotide- binding oligomerization domain, leucine-rich repeat and pyrin domain containing nod-like receptor family protein 3 (NLRP3), IL-1β, caspase-1, and gasdermin D (GSDMD) in myocardial tissue cells. Salvianolic acid B and notoginsenoside R1 protected H9C2 cardiomyocytes from hypoxia and reoxygenation injury and reduced the levels of tumor necrosis factor α (TNF-α) and IL-6 in the cell supernatant, decreasing the NLRP3, IL-18, IL-1β, caspase-1, and GSDMD expression in H9C2 cardiomyocytes. GXDSF can reduce the myocardial infarction area and alleviate the damage to myocardial structure in rats with MIRI, which may be related to the regulation of the NLRP3.
Conclusions:
GXDSF reduces MIRI in rat myocardial infarction injury, improves structural damage in myocardial ischemia injury, and reduces myocardial tissue inflammation and oxidative stress by lowering inflammatory factors and controlling focal cell death signaling pathways.
Insights
Guanxin Danshen formula (GXDSF) protects against myocardial ischemia/reperfusion injury (MIRI) in rats. It reduces inflammation and cell death by targeting the NLRP3 inflammasome pathway, preserving heart tissue.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Cellular Biology
Background:
- Myocardial ischemia/reperfusion injury (MIRI) causes significant myocardial tissue necrosis, increasing infarct size.
- Understanding protective mechanisms against MIRI is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the protective effects of Guanxin Danshen formula (GXDSF) on MIRI in a rat model.
- To elucidate the underlying mechanism of GXDSF's cardioprotective action.
Main Methods:
- Establishment of MIRI model in rats and a H9C2 cardiomyocyte hypoxia-reoxygenation injury model.
- Assessment of cardiac function, myocardial structural integrity, serum inflammatory markers, and oxidative stress indicators.
- Analysis of key protein expression, including NLRP3 inflammasome components (NLRP3, caspase-1, GSDMD) and cytokines (IL-1β, IL-6, TNF-α).
Main Results:
- GXDSF significantly reduced myocardial infarct size and structural damage in MIRI rats.
- Treatment decreased serum levels of IL-1β and IL-6, myocardial enzyme activity, and increased superoxide dismutase (SOD) activity.
- GXDSF suppressed the expression of NLRP3, IL-1β, caspase-1, and GSDMD in myocardial tissue and H9C2 cells.
Conclusions:
- GXDSF demonstrates significant cardioprotective effects against MIRI in rats.
- The formula alleviates myocardial inflammation and oxidative stress by modulating the NLRP3 inflammasome pathway.
- GXDSF improves myocardial structure and reduces infarct size, offering a potential therapeutic strategy for MIRI.
