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Related Experiment Video

Updated: Jun 23, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Spatially Addressable Multiplex Biodetection by Calibrated Micro/Nanostructured Surfaces.

Serban Dobroiu^1,2, Falco C M J M van Delft^3,4, Ayyappasamy Sudalaiyadum Perumal²

¹Department of Electrical Engineering & Electronics, University of Liverpool, Liverpool L69 3GJ, U.K.

|April 26, 2023

Summary

This summary is machine-generated.

This study introduces microstructured domes to enhance fluorescence interference contrast (FLIC) biosensing, enabling sensitive detection of low analyte concentrations by creating distinct fluorescent rings for improved signal specificity.

Keywords:

diagnostic devices fluorescence interference contrast microarrays microstructures nanostructures

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Area of Science:

Biophotonics
Nanotechnology
Biosensing

Background:

Biosensing technologies face challenges in detecting low analyte concentrations.
Fluorescence interference contrast (FLIC) enhances sensitivity but is highly sensitive to wavelength and vertical position.
Existing FLIC methods can suffer from signal suppression due to minor variations.

Purpose of the Study:

To introduce quasi-circular lenticular microstructured domes as continuous-mode optical filters for FLIC.
To overcome the wavelength sensitivity limitations of traditional FLIC.
To enable simultaneous separation of fluorescent patterns for diverse fluorophore wavelengths.

Main Methods:

Fabrication of microstructures with stepwise or continuous-slope dome geometries.
Utilizing lenticular structures to modulate fluorescence intensity and lateral position.
Simulation and experimental validation using fluorescent dyes and stimulated emission depletion (STED) microscopy.

Main Results:

Lenticular microstructures generated concentric fluorescent rings with diameters determined by fluorescence wavelengths.
Shallow sloping side walls of the domes enabled simultaneous separation of fluorescent patterns across various wavelengths.
FLIC effects induced by microstructures were confirmed experimentally, demonstrating high sensitivity.

Conclusions:

The developed microstructured domes offer a novel approach to spatially addressable FLIC.
This technique significantly improves the specificity and sensitivity of fluorescence-based biosensing.
Validated for detecting SARS-CoV-2 receptor-binding domain (RBD), showing diagnostic potential.