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Updated: Aug 1, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
A survey on algorithms to characterize transcription factor binding sites
Manuel Tognon1,2,3, Rosalba Giugno1, Luca Pinello2,3,4
1Computer Science Department, University of Verona, Verona, Italy.
This review covers experimental and computational methods for identifying transcription factor binding sites (TFBS). It highlights current techniques, their pros and cons, and future directions in motif discovery.
Area of Science:
- Bioinformatics
- Molecular Biology
- Genomics
Background:
- Transcription factors (TFs) regulate gene expression by binding to specific DNA sequences.
- Identifying these transcription factor binding sites (TFBS) is crucial for understanding cellular regulation.
- Numerous experimental and computational approaches have been developed for TFBS motif discovery.
Conclusions:
- A thorough understanding of TFBS is essential for deciphering gene regulation.
- The field benefits from a combination of experimental and computational strategies.
- Future research should focus on addressing current limitations and exploring novel approaches for motif discovery.
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