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Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing
Sylvia Merkert1, Alexandra Haase2, Julia Dahlmann1
1Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany.
Researchers created NRF2-knockout human iPS cells to study its role in viral infections. These NRF2-deficient cells are valuable tools for testing antiviral compounds that target the NRF2 pathway.
Area of Science:
- Cell Biology
- Virology
- Immunology
Background:
- Nuclear factor erythroid 2-related factor 2 (NRF2) is a key regulator of cellular antioxidant and anti-inflammatory responses.
- Understanding NRF2's role in viral infections is crucial for developing novel therapeutic strategies.
Purpose of the Study:
- To generate NRF2-knockout human induced pluripotent stem cells (iPSCs) for studying viral pathogenesis.
- To create a cellular model for evaluating antiviral compounds targeting the NRF2 signaling pathway.
Main Methods:
- CRISPR/Cas9 gene editing was employed to create two NRF2 knockout iPSC clones (MHHi001-A-6 and MHHi001-A-7) from the MHHi001-A human iPSC line.
- Induced pluripotent stem cells were differentiated into epithelial and endothelial cells.
Main Results:
- Successfully generated NRF2-knockout human iPSC clones.
- The resulting NRF2-deficient cells provide a platform for investigating NRF2's impact on viral infections.
- These cells can be utilized to assess the efficacy of NRF2 pathway activators as antiviral agents.
Conclusions:
- NRF2-knockout iPSC-derived cells are valuable tools for studying the role of NRF2 in viral infections.
- This research facilitates the development and testing of new antiviral therapies targeting the NRF2 pathway.
