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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Cellular barcoding tracks heterogeneous clones through selective pressures and phenotypic transitions.

Kennedy K Howland¹, Amy Brock¹

¹Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78734, USA.

Trends in Cancer

|April 27, 2023

Summary

Genomic DNA barcoding combined with single-cell transcriptomics tracks cancer cell clones. This approach reveals how tumor cell heterogeneity, selection, and plasticity drive cancer progression and treatment response.

Keywords:

barcoding drug resistance phenotypic plasticity tumor evolution tumor heterogeneity

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Area of Science:

Cancer research
Genomics
Molecular biology

Background:

Cancer cell populations are heterogeneous.
Understanding clonal dynamics is crucial for cancer progression and treatment.
Genomic DNA barcoding offers a method to track clonal cell fates.

Purpose of the Study:

To explore the application of genomic DNA barcoding coupled with single-cell transcriptomics.
To enable longitudinal analysis of molecular mechanisms at the clone level.
To investigate tumor cell heterogeneity, selection, and phenotypic plasticity in cancer.

Main Methods:

Genomic DNA barcoding for clonal subpopulation tracking.
Single-cell transcriptomics for molecular profiling.
Longitudinal analysis of cellular barcoding and transcriptomic data.

Main Results:

Demonstrated the capability of genomic DNA barcoding to measure clonal subpopulation dynamics.
Enabled detailed, clone-level resolution of molecular mechanisms.
Facilitated tracking of clonal cell states during cancer progression and treatment response.

Conclusions:

Genomic DNA barcoding and single-cell transcriptomics are powerful tools for studying cancer.
These technologies provide new opportunities to address fundamental questions in cancer biology.
Insights into tumor heterogeneity, selection, and plasticity can be gained.