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Related Experiment Video

Updated: Aug 1, 2025

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

High- and Super-Resolution Imaging of Cell-Cell Interfaces.

Julia Sajman^1,2, Eilon Sherman³

¹Racah Institute of Physics, The Hebrew University, Jerusalem, Israel.

Methods in Molecular Biology (Clifton, N.J.)

|April 27, 2023

Summary

This summary is machine-generated.

Researchers developed a new method to align cell-cell interfaces for high-resolution imaging. This technique enables detailed visualization of crucial cellular interactions, like immune synapses, using advanced microscopy.

Keywords:

Cell-to-cell interaction Diffraction limit Imaging Immune synapse Microcopy Super-resolution T cells

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Last Updated: Aug 1, 2025

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Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

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Published on: February 11, 2015

Area of Science:

Cell Biology
Microscopy
Immunology

Background:

Physical interfaces are critical for cell-cell interactions and function.
High-resolution optical imaging of these interfaces is challenging due to alignment issues.
Existing methods struggle to achieve optimal interface orientation for imaging.

Purpose of the Study:

To develop a simple and robust method for aligning cell-cell interfaces parallel to the imaging plane.
To enable aberration-free, high-resolution imaging of diverse cell-cell interactions.
To overcome limitations in visualizing the intricate structures within cellular interfaces.

Main Methods:

Adhering interacting cells to two opposing coverslips.
Bringing cells into controlled and stable contact.
Utilizing various microscopy techniques including bright-field, confocal, STED, and dSTORM.

Main Results:

Demonstrated successful alignment of cell-cell interfaces parallel to the coverslip.
Achieved aberration-free, high-resolution imaging of immune synapses (T cell-APC interfaces).
Showcased the method's compatibility with multiple advanced imaging techniques.

Conclusions:

The presented method provides a simple and widely applicable approach for high-resolution imaging of cell-cell interfaces.
Enables detailed study of the structure and molecular organization within various cellular interfaces.
Significantly advances the capability to visualize and understand cell-cell communication.