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Updated: Aug 1, 2025
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
Published on: June 1, 2019
Isolation of the B Cell Immune Synapse for Proteomic Analysis
Diogo M Cunha1,2, Sara Hernández-Pérez1,2, Pieta K Mattila3,4
1Institute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland.
Abstract:
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
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