Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

19.5K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
19.5K
In-situ Hybridization02:31

In-situ Hybridization

9.2K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GlycoAvatars: bead-coated membrane models for studying the cancer-immune cells interactome.

Communications biology·2026
Same author

B cell mechanotransduction via ATAT1 coordinates actin and lysosomal dynamics at the immune synapse.

The Journal of cell biology·2025
Same author

Fusion of Complement Fragment C3d Enhances Germinal Center Responses to HIV-1 Envelope Glycoproteins.

bioRxiv : the preprint server for biology·2025
Same author

Enhanced antibody responses in CD19-Cre mice.

Scientific reports·2025
Same author

Endolysosomal vesicles at the center of B cell activation.

The Journal of cell biology·2024
Same author

The Small GTPase Rab7 Regulates Antigen Processing in B Cells in a Possible Interplay with Autophagy Machinery.

Cells·2023

Related Experiment Video

Updated: May 24, 2025

Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

8.9K

High-Resolution Imaging of Intracellular Trafficking of B Cell Receptor Using Specific Hybridization Internalization

Sara Hernández-Pérez1,2,3,4, Pieta K Mattila5,6,7

  • 1Institute of Biomedicine, and MediCity Research Laboratories, University of Turku, Turku, Finland. rekgshe@ucl.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2025
PubMed
Summary

Researchers developed a new microscopy method to track B cell receptor (BCR) internalization. This technique enhances visualization of intracellular processes by specifically detecting internalized BCRs, overcoming challenges posed by surface-bound receptors.

Keywords:
B cellsBCRInternalizationMicroscopyTrafficVesicle

More Related Videos

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.8K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.7K

Related Experiment Videos

Last Updated: May 24, 2025

Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

8.9K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.8K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.7K

Area of Science:

  • Cell Biology
  • Immunology
  • Microscopy

Background:

  • B cells play a crucial role in immune responses.
  • Visualizing intracellular traffic, especially B cell receptor (BCR) internalization, is challenging due to high surface receptor density.
  • Distinguishing internalized BCRs from surface-bound BCRs is difficult with conventional microscopy.

Purpose of the Study:

  • To adapt the Specific Hybridization Internalization Probe (SHIP) assay for microscopy-based studies of BCR internalization.
  • To develop a method for unambiguous detection of internalized BCRs in B cells.
  • To enhance the precision of imaging intracellular processes.

Main Methods:

  • Adaptation of the Specific Hybridization Internalization Probe (SHIP) assay for microscopy.
  • Utilizing single-stranded DNA (ssDNA) fluorescence internalization probes (FIPs) and complementary ssDNA quenching probes.
  • Application to various imaging modalities, including live-cell and super-resolution microscopy.

Main Results:

  • The adapted SHIP assay enables clear visualization of internalized BCRs.
  • The method effectively distinguishes intracellular BCRs from extracellular, surface-bound BCRs.
  • The assay demonstrates versatility across different microscopy techniques.

Conclusions:

  • The SHIP assay is a valuable tool for studying BCR internalization using microscopy.
  • This method significantly improves the precision of detecting internalized BCRs.
  • Enhanced imaging of intracellular processes in B cells is now feasible.