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Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
Nagaja Capitani1, Chiara Cassioli2, Keerthana Ravichandran3
1Department of Life Sciences, University of Siena, Siena, Italy. capitani2@unisi.it.
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The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.
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