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Updated: Aug 1, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Detection of Genes Encoding Microbial Surface Component Recognizing Adhesive Matrix Molecules in
Mohammed Alorabi1, Uroosa Ejaz2, Bahram Khan Khoso3
1Department of Biotechnology, College of Sciences, Taif University, Taif 21944, Saudi Arabia.
Abstract:
Pyoderma is a common skin infection predominantly caused by Staphylococcus aureus. In addition to methicillin resistance, this pathogen is resistant to many other antibiotics, which ultimately limits the available treatment options. Therefore, the present study aimed to compare the antibiotic-resistance pattern, to detect the mecA gene and the genes encoding microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) in S. aureus isolates. A total of 116 strains were isolated from patients suffering with pyoderma. Disk diffusion assay was opted to perform antimicrobial susceptibility testing of the isolates. Out of the isolates tested, 23-42.2% strains appeared susceptible to benzylpenicillin, cefoxitin, ciprofloxacin and erythromycin. While linezolid was found to be the most effective anti-staphylococcal drug, followed by rifampin, chloramphenicol, clindamycin, gentamicin and ceftaroline. Out of 116 isolates, 73 (62.93%) were methicillin-resistant S. aureus (MRSA). Statistically significant (p ≤ 0.05) differences in antibiotic resistance patterns between MRSA and methicillin-susceptible S. aureus (MSSA) were found. A significant association of resistance to ceftaroline, rifampin, tetracycline, ciprofloxacin, clindamycin, trimethoprim-sulfamethoxazole and chloramphenicol was found in MRSA. However, no significant difference was observed between MRSA and MSSA for resistance against gentamicin, erythromycin or linezolid. All cefoxitin-resistant S. aureus, nonetheless, were positive for the mecA gene. femA was found in all the MRSA isolates. Among other virulence markers, bbp and fnbB were found in all the isolates, while can (98.3%), clfA and fnbA (99.1%) were present predominately in MRSA. Thus, this study offers an understanding of antibiotic resistance MSCRAMMs, mecA, and femA gene patterns in locally isolated strains of S. aureus.
Insights
This study investigated antibiotic resistance in Staphylococcus aureus causing pyoderma. Methicillin-resistant S. aureus (MRSA) showed significant resistance to various antibiotics, with linezolid being the most effective drug.
Area of Science:
- Microbiology
- Dermatology
- Infectious Diseases
Background:
- Pyoderma is a common skin infection often caused by Staphylococcus aureus.
- Antibiotic resistance in S. aureus, particularly methicillin resistance, limits treatment options.
- Understanding resistance patterns and virulence factors is crucial for effective management.
Purpose of the Study:
- To compare antibiotic resistance patterns in S. aureus isolates from pyoderma patients.
- To detect the presence of the mecA gene and genes encoding microbial surface component recognizing adhesive matrix molecules (MSCRAMMs).
- To analyze the association between resistance and specific virulence genes in MRSA and MSSA.
Main Methods:
- Antimicrobial susceptibility testing using disk diffusion assay on 116 S. aureus strains.
- Detection of mecA, femA, and various MSCRAMM genes (bbp, fnbB, can, clfA, fnbA).
- Statistical analysis to compare resistance patterns between MRSA and MSSA.
Main Results:
- 62.93% of isolates were methicillin-resistant S. aureus (MRSA).
- Linezolid was the most effective antibiotic, followed by rifampin and chloramphenicol.
- MRSA strains showed significant resistance to ceftaroline, rifampin, tetracycline, ciprofloxacin, clindamycin, trimethoprim-sulfamethoxazole, and chloramphenicol.
- The mecA gene was present in all cefoxitin-resistant isolates, and femA in all MRSA isolates.
- Specific MSCRAMM genes (bbp, fnbB, can, clfA, fnbA) were prevalent, particularly in MRSA.
Conclusions:
- The study highlights significant antibiotic resistance in local S. aureus strains causing pyoderma, especially in MRSA.
- Understanding the genetic basis of resistance and virulence is essential for developing targeted therapies.
- Linezolid and rifampin show promise as effective treatments against resistant S. aureus.
