Optimization of Phenolic Compounds Extraction and Antioxidant Activity from Inonotus hispidus Using...

Area of Science:

• Natural Product Chemistry
• Food Science
• Biotechnology

Background:

• Conventional extraction methods for bioactive compounds can be inefficient.
• Ultrasound-assisted extraction (UAE) offers a promising alternative for enhanced compound recovery.
• Mushrooms like Inonotus hispidus are rich sources of bioactive compounds with antioxidant properties.

Purpose of the Study:

• To optimize ultrasound-assisted extraction (UAE) conditions for maximizing total polyphenol content (TPC) and antioxidant activity of Inonotus hispidus.
• To evaluate the efficacy of different solvents (ethanol and methanol) in the UAE process.
• To identify the primary phenolic compounds responsible for the antioxidant capacity.

Main Methods:

• Ultrasound-assisted extraction (UAE) was employed to extract bioactive compounds from Inonotus hispidus.
• Response Surface Methodology (RSM) was utilized to optimize extraction parameters (solvent concentration, solvent-to-solid ratio, extraction time).
• Total polyphenol content (TPC) was determined, and antioxidant capacity was assessed using DPPH scavenging capacity and FRAP assays.

Main Results:

• Ethanolic extracts (40% v/v) demonstrated significantly higher TPC and antioxidant activity compared to methanolic extracts.
• Optimized UAE conditions involved 40% ethanol, a 75 mL/g ratio, and 20 min extraction time.
• Hispidin and its analogs were identified as the predominant phenolic compounds, contributing significantly to the extract's antioxidant potential.

Conclusions:

• UAE is an effective method for extracting high-value antioxidant compounds from Inonotus hispidus.
• The optimized extraction process provides a foundation for the industrial, pharmaceutical, and food applications of this mushroom's bioactive compounds.
• Inonotus hispidus represents a valuable natural source of potent antioxidant compounds.