Strategies for enzyme isolation using dye-ligand and related adsorbents

Approaches to the use of a large range of dye-ligand and similar adsorbents for protein isolation are described. The adsorbents behave in a similar fashion relative to each other with a variety of different applied protein mixtures, so a system of dividing the adsorbents into five groups according to their protein-binding ability has been adopted. In this way it becomes unnecessary to screen all dyes, and an ideal selection of dyes and conditions can quickly be achieved.