Batch separation of uracil derivatives from urine

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Uracil derivatives are important biomarkers in urine.
• Accurate and rapid methods for their extraction and quantification are needed.

Purpose of the Study:

• To develop a rapid batch extraction method for urine uracil derivatives.
• To enable both spectrophotometric screening and HPLC quantification.

Main Methods:

• Modified deionizing resins (sulfonic acid and acetate forms) were used for batch extraction.
• Uracil and pseudouridine were quantified using HPLC on reversed-phase columns.
• Spectrophotometric assay based on spectral shift in alkaline conditions was employed.

Main Results:

• A rapid extraction process (<15 min) was established.
• Spectrophotometric conversion factors were determined for uracil (0.0046) and pseudouridine (0.0035).
• Pseudouridine levels in test urines were measured at 24 and 19.3 nmol/μmol creatinine.

Conclusions:

• The developed method provides a fast and accurate approach for analyzing urine uracil derivatives.
• This technique is suitable for both clinical screening and precise quantification.