Phosphate-driven H₂O₂ decomposition on DNA-bound bio-inspired activated carbon-based sensing platform for biological...

Hydrogen peroxide (H₂O₂) is one of the most important reactive oxygen species (ROS). Increased endogenous H₂O₂ levels indicate oxidative stress and could be a potential marker of many diseases, including Alzheimer's, cardiovascular diseases, and diabetes. However, consuming H₂O_2-incorporated food has adverse effects on humans and is a serious health concern. We used salmon testes DNA with bio-inspired activated carbon (AC) as an electrocatalyst for developing a novel H₂O₂ sensor. The phosphate backbone of DNA contains negatively charged oxygen groups that specifically attract protons from H₂O₂ reduction. We observed a linearity range of 0.01-250.0 μM in the H₂O₂ reduction peak current with a detection limit of 2.5 and 45.7 nM for chronoamperometric and differential pulse voltammetric studies. High biocompatibility of the sensor was achieved by the DNA, facilitating endogenous H₂O₂ detection. Moreover, this non-enzymatic sensor could also help in the rapid screening of H₂O₂-contaminated foods.