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Updated: Aug 1, 2025
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Published on: April 21, 2016
A novel 3D bio-printing "liver lobule" microtissue biosensor for the detection of AFB1
Lifeng Wang1, Hanwen Cao1, Hui Jiang2
1College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, Jiangsu 210023, PR China.
Abstract:
In this paper, a novel "liver lobule" microtissue biosensor based on 3D bio-printing is developed to rapidly determine aflatoxin B1 (AFB1). Methylacylated Hyaluronic acid (HAMA) hydrogel, HepG2 cells, and carbon nanotubes are used to construct "liver lobule" models. In addition, 3D bio-printing is used to perform high-throughput and standardized preparation in order to simulate the organ morphology and induce functional formation. Afterwards, based on the electrochemical rapid detection technology, a 3D bio-printed "liver lobule" microtissue is immobilized on the screen-printed electrode, and the mycotoxin is detected by differential pulse voltammetry (DPV). The DPV response increases with the concentration of AFB1 in the range of 0.1-3.5 μg/mL. The linear detection range is 0.1-1.5 μg/mL and the calculated lowest detection limit is 0.039 μg/mL. Thus, this study develops a new mycotoxin detection method based on the 3D printing technology, which has high stability and reproducibility. It has wide application prospects in the field of detection and evaluation of food hazards.
