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Methods of Sterilization II: Chemical Methods01:30

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
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Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
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Inactivating Salmonella Enteritidis on shell eggs by using ozone microbubble water.

Chia-Min Lin1, Song-Yue Chen1, Yi-Ting Lin1

  • 1Department of Seafood Science, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan.

International Journal of Food Microbiology
|April 30, 2023
PubMed
Summary

Ozone microbubble water effectively sanitizes Salmonella Enteritidis on shell eggs. This novel method significantly reduces bacteria in large water volumes without affecting egg quality.

Keywords:
Ozone microbubbleSalmonellaShell eggs

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Area of Science:

  • Food Science
  • Microbiology
  • Water Treatment Technologies

Background:

  • Salmonella Enteritidis is a major pathogen on shell eggs.
  • Chlorine washing is the standard sanitization method.
  • Microbubble technology offers a novel alternative for large-scale applications.

Purpose of the Study:

  • To evaluate the efficacy of ozone microbubble (OMB) water for disinfecting S. Enteritidis on shell eggs.
  • To assess OMB disinfection in large water quantities.
  • To determine the impact of OMB treatment on egg sensory quality.

Main Methods:

  • Eggs spiked with S. Enteritidis were treated with OMB water generated by Nikuni and Calpeda systems.
  • Disinfection efficacy was tested at various activation times and washing durations.
  • Treatments included controls: unwashed, water washing, ozone only, and microbubble only.
  • Sensory quality was assessed after 15-day storage.

Main Results:

  • The highest reduction of 5.19 log CFU/egg was achieved with 20-min activation and 60-s washing.
  • Effective S. Enteritidis reduction (3.07-4.32 log CFU/egg) was observed in large water volumes (25-100 L).
  • OMB treatment did not diminish sensory characteristics of eggs compared to unwashed controls.

Conclusions:

  • Ozone microbubble water is a highly effective disinfectant for S. Enteritidis on shell eggs, even in large water volumes.
  • This method presents a promising alternative to traditional chlorine washing.
  • OMB treatment maintains the sensory quality of shell eggs.