Phenolic compounds interfere in the Ampliflu Red/peroxidase assay for hydrogen peroxide

Methods employing horseradish peroxidase (HRP) are popular for quantification of hydrogen peroxide. This communication reports interference of the Ampliflu Red-HRP assay by phenolic compounds, abundant in food and beverages of plant origin. Concentrations of catechin, propyl gallate, quercetin and gallic acid lowering the yield of the product, resorufin, in this system by 50% were lower than 10 μM. The extent of inhibition increased with decreasing hydrogen peroxide concentration. These results point to the necessity of a careful interpretation of results concerning the quantification of hydrogen peroxide in materials containing phenolic compounds with methods employing HRP, especially when low concentrations of hydrogen peroxide are concerned.