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Updated: Jul 31, 2025
07:25
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Published on: June 9, 2020
9.6K
Harnessing CRISPR-Cas for oomycete genome editing
Jochem N A Vink1, Max Hayhurst1, Monica L Gerth2
1School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.
Trends in Microbiology
|May 1, 2023
Summary
Genome editing in oomycetes is advancing rapidly with clustered regularly interspaced short palindromic repeats (CRISPR)-Cas technologies. These tools are crucial for understanding oomycete pathogens and developing disease management strategies.
Area of Science:
- Microbiology
- Genetics
- Plant Pathology
Background:
- Oomycetes are significant plant and animal pathogens causing global devastation.
- Genome editing tools for oomycetes lag behind those for other microorganisms.
Purpose of the Study:
- To review recent advancements in CRISPR-Cas technologies for oomycete genome editing.
- To discuss challenges and solutions for applying these tools in oomycetes.
Main Methods:
- Review of CRISPR-Cas9, CRISPR-Cas12a, ribonucleoprotein (RNP) delivery, and complementation techniques.
- Analysis of current challenges and potential strategies for oomycete genome editing.
Main Results:
- CRISPR-Cas technologies are expanding the genome editing toolbox for oomycetes.
- New methods offer improved efficiency and versatility for genetic manipulation.
Conclusions:
- CRISPR-Cas advancements are illuminating oomycete biology.
- These tools are essential for developing effective oomycete disease management strategies.
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