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GhbZIP30-GhCCCH17 module accelerates corm dormancy release by reducing endogenous ABA under cold storage in
Jia-Hui Liang1,2, Jing-Ru Li1, Chang Liu1
1Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing, China.
Plant, Cell & Environment
|May 2, 2023
Summary
Long-term cold treatment breaks gladiolus corm dormancy by increasing sugars and decreasing abscisic acid. A GhbZIP30-GhCCCH17 module mediates this process, regulating ABA metabolism for off-season flower production.
Area of Science:
- Plant Science
- Molecular Biology
- Horticulture
Background:
- Gladiolus hybridus corm dormancy limits off-season production.
- Long-term cold treatment (LT) promotes corm dormancy release (CDR) by increasing sugars and reducing abscisic acid (ABA).
Purpose of the Study:
- To identify the molecular mechanism underlying sugar and ABA antagonism during Gladiolus CDR.
- To elucidate the role of the GhbZIP30-GhCCCH17 module in mediating CDR.
Main Methods:
- Yeast one-hybrid, Dual-LUC, ChIP-qPCR, and EMSA to confirm GhbZIP30 binding to GhCCCH17 promoter.
- Gene silencing and overexpression to assess the functions of GhbZIP30 and GhCCCH17.
- Analysis of ABA metabolic genes in response to treatments.
Main Results:
- Sugars promote Gladiolus CDR by reducing ABA levels.
- GhbZIP30 directly activates GhCCCH17 transcription, forming a GhbZIP30-GhCCCH17 module.
- This module positively responds to LT, sugars, and cytokinin, and regulates ABA metabolism.
Conclusions:
- The GhbZIP30-GhCCCH17 module is crucial for cold- and glucose-induced CDR in Gladiolus.
- This module functions by modulating ABA metabolic genes, offering a target for enhancing off-season flower production.
