Utility of Gene Expression Profiling in Skin Cancer: A Comprehensive Review.

Area of Science:

• Oncology
• Genomics
• Molecular Biology

Background:

• Metastatic potential in skin cancer necessitates advanced diagnostic tools.
• Gene expression profiling (GEP) offers innovative insights into skin cancer tumor biology.
• Current methods rely on ribonucleic acid (RNA) transcript quantification.

Approach:

• Utilizes reverse transcriptase-polymerase chain reaction to convert RNA to deoxyribonucleic acid (DNA) for quantification.
• Incorporates RNA-sequencing (RNA-seq) for measuring known sequences and identifying novel genes.
• Requires minimal RNA input and demonstrates high reproducibility.

Key Points:

• GEP aids in understanding skin cancer's metastatic potential.
• RNA-seq expands genomic knowledge by detecting novel genes.
• Several GEPs are developed to improve skin cancer diagnosis and prognosis.

Conclusions:

• GEP is a valuable tool for advancing skin cancer research and clinical application.
• The technology enhances the understanding of skin cancer biology, aiding in patient management.
• Ongoing investigations aim to further integrate GEP into clinical practice for improved outcomes.