[Change in cellular pH in hyperthermia and hypoxia]

Change of pH of cultivated cells (Chinese hamster fibroblasts and pig embryonic kidney cells) was studied during their incubation under oxygenation conditions and at different temperatures using microfluorimetry with fluorescein diacetate. It was shown that pHc was 7.0 at temperatures 22-37 degrees C. Hyperthermia and hypoxia resulted in cell acidity. The effect was enhanced with an increase in the period of exposure to both agents. Intracellular pH more acid values were noted in the effect of hyperthermia in the presence of hypoxia or exogenous pH acid values. The authors made a conclusion of the importance of a degree of acidity of the intracellular medium for the survival of cells irradiated under hyperthermia conditions.