Direct preparation and G-banding of chromosomes of mouse liver cells

A method has been developed for the direct preparation and G-banding of chromosomes of mouse liver cells by combining the techniques of liver perfusion and preparation of G-banded chromosomes with partial hepatectomy and colcemid treatment of the animal. The results indicate that cytogenetic investigations of isolated preneoplastic liver cells are possible. The method offers an increased possibility for the use of the liver as an in vivo test system for mutagens and carcinogens.