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RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jul 31, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Recursive splicing discovery using lariats in total RNA sequencing.

Emma R Hoppe^1,2,3, Dylan B Udy^1,2, Robert K Bradley^4,2,3

¹Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.

Life Science Alliance

|May 3, 2023

Summary

This summary is machine-generated.

Recursive splicing, a unique gene expression process, was found in more human intron types than previously known. This mechanism influences gene regulation and the creation of varied protein forms.

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Last Updated: Jul 31, 2025

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Area of Science:

Molecular Biology
Genetics
Transcriptomics

Background:

Recursive splicing is a non-canonical intron removal process involving multiple steps.
Limited knowledge exists regarding the full extent and regulatory roles of recursive splicing in the human genome.

Purpose of the Study:

To identify novel recursive splice sites within human constitutive introns and alternative exons.
To investigate the prevalence, characteristics, and potential regulatory functions of recursive splicing.

Main Methods:

Employed an unbiased approach utilizing intron lariats to detect recursive splice sites.
Analyzed the human transcriptome for evidence of recursive splicing across various intron sizes and exon types.

Main Results:

Discovered recursive splicing in a wider spectrum of intron sizes than previously documented.
Identified a new site for recursive splicing at the distal ends of cassette exons.
Found evidence for the conservation of these sites in higher vertebrates and their role in alternative exon exclusion.

Conclusions:

Recursive splicing is more prevalent in the human transcriptome than previously understood.
This mechanism, particularly at cassette exon ends, can influence alternative splicing and gene expression.
The findings highlight the significance of recursive splicing in generating transcript diversity.