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Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...

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Updated: Jul 31, 2025

Cell Population Analyses During Skin Carcinogenesis

Cell Population Analyses During Skin Carcinogenesis

Published on: August 21, 2013

Single-Cell and Spatial Transcriptomic Analysis of Human Skin Delineates Intercellular Communication and Pathogenic

Kim Thrane¹, Mårten C G Winge², Hongyu Wang³

¹Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Solna, Sweden.

The Journal of Investigative Dermatology

|May 4, 2023

Summary

This summary is machine-generated.

Researchers identified a unique human skin cell subpopulation involved in heavy metal processing, which is expanded in skin diseases like psoriasis. This finding offers new insights into epidermal homeostasis and potential therapeutic targets for skin conditions.

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Cell Population Analyses During Skin Carcinogenesis

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Area of Science:

Dermatology and Molecular Biology
Investigating the molecular mechanisms of skin homeostasis and disease.
Utilizing single-cell and spatial transcriptomics for high-resolution skin tissue analysis.

Background:

Epidermal homeostasis relies on keratinocyte balance, but cross-species mechanisms and disease links are unclear.
Understanding species-specific differences in skin biology is crucial for deciphering disease pathogenesis.
Existing knowledge gaps hinder the development of targeted therapies for skin disorders.

Purpose of the Study:

To elucidate conserved and divergent mechanisms of epidermal homeostasis across species.
To identify novel cell subpopulations and pathways contributing to skin diseases.
To create a valuable resource for skin research.

Main Methods:

Integrated human and mouse skin single-cell RNA sequencing and spatial transcriptomics data.
Improved human skin cell-type annotation using spatial transcriptomics.
Performed cross-species comparative analyses and cell-of-origin enrichment analysis.

Main Results:

Identified a human-specific spinous keratinocyte subpopulation with proliferative capacity and heavy metal processing functions.
This subpopulation was expanded in psoriasis and zinc-deficiency dermatitis, suggesting disease relevance.
Nominated pathogenic cell subpopulations and communication pathways in genodermatoses, revealing potential therapeutic targets.

Conclusions:

A novel human skin cell subpopulation plays a role in epidermal homeostasis and disease.
Dysfunction of specific cell subpopulations may be a hallmark of skin diseases.
The integrated dataset and web resource will advance mechanistic and translational skin research.