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Updated: Jul 31, 2025

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Published on: November 18, 2019

Evaluating Cellular Viability by iTRAQ Proteomic Profiling.

Anne Poljak¹, Mark Raftery¹, Patsie Polly²

¹Bioanalytical Mass Spectrometry Facility (BMSF), Mark Wainwright Analytical Centre (MWAC), University of New South Wales, Sydney, NSW, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|May 4, 2023

Summary

This summary is machine-generated.

Cellular proteomes and metabolomes reflect cell health and viability. Proteomic analysis, particularly using isobaric tags for relative and absolute quantification (iTRAQ), quantifies these changes for insights into aging and disease.

Keywords:

Cell viability Isobaric tags for relative and absolute quantification (iTRAQ)Mass spectrometry Proteomics

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Area of Science:

Biochemistry
Cell Biology
Proteomics

Background:

Cellular health, function, and viability are influenced by proteomes and metabolomes.
These
omic
profiles dynamically change to maintain homeostasis and respond to stimuli.
Proteomic
fingerprints
offer insights into cellular aging, disease responses, and environmental adaptations.

Purpose of the Study:

To explore the significance of proteomic and metabolomic profiles in reflecting cellular status.
To introduce quantitative proteomic methods for analyzing cellular changes.
To focus on the isobaric tags for relative and absolute quantification (iTRAQ) method for proteomic analysis.

Main Methods:

Proteomic analysis to determine qualitative and quantitative changes.
Utilizing labeling methods for precise measurement.
Focusing on isobaric tags for relative and absolute quantification (iTRAQ) for identifying and quantifying proteomic expression.

Main Results:

Proteomic and metabolomic profiles provide a comprehensive view of cellular health and viability.
Dynamic changes in these profiles are crucial for maintaining cellular homeostasis.
iTRAQ enables accurate quantification of proteomic expression changes in cells and tissues.

Conclusions:

Proteomic and metabolomic analyses are vital for understanding cellular responses and viability.
The iTRAQ method is a powerful tool for quantitative proteomic studies.
Understanding proteomic changes aids in diagnosing and managing conditions like aging and disease.