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Updated: Jun 9, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
Published on: April 22, 2013
The phase range extension and accuracy improvement in Fresnel biprism-based digital holography microscopy
Hasti Nourzadeh1, Maryam Lotfi1, Humberto Cabrera2
1Department of Physics, Shahid Beheshti University, Evin, Tehran 19839-69411, Iran.
Abstract:
We report a highly stable and affordable dual-wavelength digital holographic microscopy system based on common-path geometry. A Fresnel biprism is used to create an off-axis geometry, and two diode laser sources with different wavelengths λ1 = 532 nm and λ2 = 650 nm generate the dual-wavelength compound hologram. In order to extend the measurement range, the phase distribution is obtained using a synthetic wavelength Λ1 = 2930.5 nm. Furthermore, to improve the system's temporal stability and reduce speckle noise, a shorter wavelength (Λ2 = 292.5 nm) is used. The feasibility of the proposed configuration is validated by the experimental results obtained with Molybdenum trioxide, Paramecium, and red blood cell specimens.

