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Updated: Jul 31, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2
Saiwei Zhang1, Xiaohua Fu2, Ya-Nan Wang3
1Shanghai Academy of Environmental Sciences, Shanghai 200233, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Abstract:
Here, we present a protocol to analyze the inhibition of self-generated extracellular free organic carbon (EFOC) on CO2 fixation by chemoautotrophic bacteria. We detail the construction and operation of membrane reactor, followed by a simulation experiment to verify the inhibition of EFOC on CO2 fixation. We further describe the analysis of main inhibitory components in EFOC and measurement of abundance and transcription level of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) gene to clarify the mechanism of the main inhibition components on CO2 fixation. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.
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