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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Published on: February 10, 2022
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Quantifying CBM-Carbohydrate Interactions Using Microscale Thermophoresis
Haiyang Wu1, Cédric Y Montanier1, Claire Dumon2
1TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2023
Abstract:
Microscale thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with a high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here we describe the application of MST in quantifying protein-carbohydrate interactions. A CBM3a and a CBM4 are titrated with insoluble substrate (cellulose nanocrystal) and soluble oligosaccharide (xylohexaose), respectively.
Keywords:
Binding studiesFluorescence quenchingFluorescent labelKd (dissociation constant)Microscale thermophoresis (MST)
