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Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of
Ruoyu Wu1, Jiaping Guo1, Minkai Wang2
1College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China.
ACS Omega
|May 8, 2023
Summary
A new fluorescent sensor detects Ochratoxin A (OTA) in food using magnetic separation and chain displacement amplification. This method offers sensitive and rapid analysis of OTA contamination, ensuring food safety.
Area of Science:
- Food Science
- Analytical Chemistry
- Nanotechnology
Background:
- Ochratoxin A (OTA) is a prevalent mycotoxin posing significant human health risks via the food chain.
- Accurate and sensitive detection methods for OTA are crucial for food safety and regulatory compliance.
Purpose of the Study:
- To develop a sensitive, specific, and rapid fluorescent sensor for Ochratoxin A detection in food samples.
- To combine magnetic separation technology with chain displacement amplification for enhanced OTA analysis.
Main Methods:
- Development of a fluorescent sensor utilizing magnetic nanomaterials for efficient target capture.
- Integration of strand displacement amplification to boost detection sensitivity.
- Application of the sensor to analyze OTA in complex food matrices like wheat, corn, and red wine.
Main Results:
- The developed sensor demonstrated excellent performance with a linearity range of 0.5-128.0 ng/mL and a low detection limit of 0.125 ng/ng/mL.
- High recoveries (85.45-107.8%) were achieved in spiked wheat, corn, and red wine samples.
- The method exhibited lower detection limits and comparable recovery rates to high-performance liquid chromatography (HPLC).
Conclusions:
- The novel fluorescent sensor provides a sensitive, specific, and efficient tool for Ochratoxin A residue analysis.
- This method is a promising alternative for routine monitoring of OTA in diverse food products.
- The combination of magnetic separation and strand displacement amplification offers advantages for complex matrix analysis.
