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Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Published on: February 16, 2017
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B Cell Profiling in Patients with Pemphigus Vulgaris.
V A Abrikosova1, Y A Mokrushina1,2, L A Ovchinnikova1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russian Federation.
Acta Naturae
|May 8, 2023
Summary
Pemphigus vulgaris patients have a high proportion of autoreactive B cells targeting desmoglein. Identifying these specific B cells could lead to more personalized therapies for this severe autoimmune disease.
Area of Science:
- Immunology
- Autoimmune Diseases
- Dermatology
Background:
- Pemphigus vulgaris is a severe autoimmune disorder characterized by autoantibodies against desmoglein 3.
- Current therapies lack selectivity, leading to non-specific B cell elimination.
- High mortality rates underscore the need for improved treatment strategies.
Purpose of the Study:
- To quantify autoreactive B cells in pemphigus vulgaris patients.
- To explore the correlation between autoantibody levels and autoreactive B cell numbers.
- To inform the development of targeted immunotherapies.
Main Methods:
- Flow cytometry to identify and quantify autoreactive B cells.
- Measurement of autoantibodies specific to desmoglein fragments.
- Correlation analysis between B cell populations and antibody titers.
Main Results:
- Autoreactive B cells constitute 0.09-0.16% of cells in pemphigus vulgaris patients.
- A positive correlation exists between autoantibody levels and the number of autoreactive B cells.
- Specific B cell populations were identified targeting various desmoglein fragments.
Conclusions:
- Pemphigus vulgaris involves a distinct population of autoreactive B cells.
- Quantifying these cells and their targets is crucial for personalized therapy.
- Targeting specific autoreactive B cells offers a promising therapeutic avenue.
