Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Deep-Learning-Enhanced Bioimaging Via Energy Traps Regulated Lanthanide Nanoparticles.

Angewandte Chemie (International ed. in English)·2026

Same author

Genetic dissection of seed quality traits in mungbean through QTL-seq of recombinant inbred line populations.

Journal of plant physiology·2026

Same author

LingGui QiHua-3 decoction (LGQH-3) ameliorates cardiorenal dysfunction in HFpEF by restoring fluid homeostasis via the PCSK6/Corin/p38 MAPK/AQPs axis.

Journal of ethnopharmacology·2026

Same author

Soft tactile chip with in-situ sensing for haptic rendering and reverse feedback enhanced gross to fine teleoperation.

Nature communications·2026

Same author

Characterization of proteoforms within α-synuclein complexes in living cells by cleavable cross-linking combined with top-down mass spectrometry.

Talanta·2026

Same author

Emissivity enhancement method for blackbody coating based on femtosecond laser direct writing.

Optics express·2026

Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026

Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026

Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026

Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026

Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026

Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 31, 2025

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Published on: August 5, 2020

Fast autofocus method for piezoelectric microscopy system for high interaction scenes.

Xiaopeng Hao¹, Bowen Zhong¹, Zhan Liao¹

¹The College of Mechanical and Electrical Engineering, Soochow University, Suzhou, China.

Microscopy Research and Technique

|May 8, 2023

Summary

This summary is machine-generated.

This study introduces a fast autofocus algorithm for piezoelectric objective drivers in microscopy. The method enhances image sharpness and vibration control, significantly improving real-time focusing speed.

Keywords:

autofocus input shaping microscope piezo objective driver

More Related Videos

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Related Experiment Videos

Last Updated: Jul 31, 2025

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Fabrication and Characterization of Thickness Mode Piezoelectric Devices for Atomization and Acoustofluidics

Published on: August 5, 2020

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Area of Science:

Microscopy
Optical Engineering
Control Systems

Background:

Piezoelectric objective driver positioners are vital in modern microscopy for their dynamic and fast response.
Achieving rapid and accurate autofocus is crucial for interactive and high-throughput microscopic systems.

Purpose of the Study:

To develop a fast autofocus algorithm for piezoelectric objective driver systems.
To enhance the real-time focusing capabilities and image acquisition speed in automated microscopy.

Main Methods:

Utilizing the Tenengrad gradient of down-sampled images to quantify image sharpness.
Employing the Brent search method for rapid convergence to the optimal focal length.
Implementing an input shaping technique to mitigate vibrations from the piezoelectric driver.

Main Results:

The proposed algorithm significantly accelerates the autofocus task for piezoelectric objective drivers.
Demonstrated improvement in the real-time focusing performance of automated microscopic systems.
Effective suppression of displacement vibrations inherent to piezoelectric actuators.

Conclusions:

The developed autofocus strategy offers high real-time performance for piezoelectric objective driver microscopy.
The integrated vibration control method is suitable for enhancing the speed and stability of automated microscopic systems.