Fast autofocus method for piezoelectric microscopy system for high interaction scenes

Piezoelectric objective driver positioners are increasingly used in the field of microscopy. They have the advantages of high dynamic and fast response. This paper presents a fast autofocus algorithm for highly interactive microscope system. First, the Tenengrad gradient of the down-sampled image is used to calculate the image sharpness, and Brent search method is adopted to quickly converge to the correct focal length. At the same time, the input shaping method is used to eliminate the displacement vibration of the piezoelectric objective lens driver and further accelerate the image acquisition speed. Experimental results show that the proposed scheme can improve the speed of the automatic focusing task of the piezoelectric objective driver and improve the real-time focus of the automatic microscopic system. HIGHLIGHTS: A high real-time autofocus strategy. A vibration control method suitable for a piezoelectric objective driver.