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Calcium Imaging in Mouse Superior Colliculus.

Zhe Li1, Ruixiang Wu1, Ya-Tang Li2

  • 1Chinese Institute for Brain Research.

Journal of Visualized Experiments : Jove
|May 8, 2023
PubMed
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This study details optical recording methods to investigate neural coding in the superior colliculus (SC). Researchers used two-photon and wide-field microscopy to visualize visual responses in mice, revealing SC processing capabilities.

Area of Science:

  • Neuroscience
  • Vision Research
  • Computational Neuroscience

Background:

  • The superior colliculus (SC) is a key midbrain visual processing center.
  • Understanding SC's neural coding is crucial for comprehending visual information processing before cortical development.

Purpose of the Study:

  • To provide a detailed protocol for optical recording of visual responses in the mouse SC.
  • To investigate whether the SC processes visual information de novo or simply inherits retinal features.

Main Methods:

  • Utilized two complementary optical recording methods: two-photon microscopy for single-cell resolution and wide-field microscopy for whole-SC imaging.
  • Employed awake mice, including a mutant with an undeveloped cortex, to study SC visual responses.
  • Detailed procedures for animal preparation, viral injection, surgery, and data acquisition/analysis.

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Main Results:

  • Two-photon calcium imaging successfully resolved visually evoked neuronal responses at the single-cell level.
  • Wide-field calcium imaging demonstrated neural activity across the entire SC.
  • Combined imaging approaches revealed SC neural coding at multiple scales.

Conclusions:

  • The presented protocol enables comprehensive investigation of neural coding in the SC.
  • Combining single-cell and whole-region imaging offers a powerful approach to study visual processing.
  • This methodology can be adapted for studying other brain regions.