Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

88
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
88
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.5K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanomechanical Analysis of Living Small Extracellular Vesicles to Identify Gastric Cancer Cell Malignancy Based on a Biomimetic Peritoneum.

ACS nano·2024
Same author

Correlative super-resolution bright-field and fluorescence imaging by microsphere assisted microscopy.

Nanoscale·2023
Same author

Skin-Inspired Capacitive Flexible Tactile Sensor with an Asymmetric Structure for Detecting Directional Shear Forces.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2023
Same author

A 4D-Printing Inverse Design Strategy for Micromachines with Customized Shape-Morphing.

Small (Weinheim an der Bergstrasse, Germany)·2023
Same author

Novel Optofluidic Imaging System Integrated with Tunable Microlens Arrays.

ACS applied materials & interfaces·2023
Same author

Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis.

Oxidative medicine and cellular longevity·2022

Related Experiment Video

Updated: Jul 31, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.6K

Self-Sensing Scanning Superlens for Three-Dimensional Noninvasive Visible-Light Nanoscale Imaging on Complex

Hao Luo1,2,3, Xiaoduo Wang1,2, Yangdong Wen4

  • 1State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.

Nano Letters
|May 8, 2023
PubMed
Summary

This study introduces a novel feedback-controlled microsphere superlens for noninvasive super-resolution imaging of complex curved surfaces. This breakthrough enables detailed nanoscale imaging of both biological and abiotic samples, expanding imaging capabilities.

Keywords:
atomic force microscopymicrospherenanoscale imagingself-sensing cantileversuper-resolutionthree-dimensional imaging

More Related Videos

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

7.8K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.9K

Related Experiment Videos

Last Updated: Jul 31, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.6K
Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

7.8K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.9K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Nanotechnology

Background:

  • Microsphere superlens technology enables label-free, real-time super-resolution imaging.
  • Current scanning methods struggle with imaging complex curved surfaces, limiting applications.

Purpose of the Study:

  • To develop a microsphere superlens capable of super-resolution imaging on complex curved surfaces.
  • To enable noninvasive, real-time, 3D nanoscale imaging of diverse samples.

Main Methods:

  • A feedback-controlled microsphere superlens system was developed.
  • A constant force was maintained between the microspheres and the sample surface.
  • Scanning imaging was performed on complex microscale surfaces.

Main Results:

  • Achieved noninvasive super-resolution optical imaging of complex abiotic and biological surfaces.
  • Simultaneously obtained three-dimensional information of the sample.
  • Demonstrated the ability to image surfaces previously inaccessible to super-resolution techniques.

Conclusions:

  • The feedback-controlled microsphere superlens overcomes limitations in imaging curved surfaces.
  • This method significantly enhances the versatility of microsphere superlens technology for various samples.
  • Promotes broader applications in nanoscale imaging of living systems and semiconductor chips.