Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering nanoparticle surface chemistry for antigen-presenting cell targeting improves specificity and safety of TLR3 agonist cancer immunotherapy.

bioRxiv : the preprint server for biology·2026
Same author

Tolerances in microfluidic master molds: a comparison of 3D printing and micromilling.

RSC advances·2026
Same author

Andexanet Alfa for Life-Threatening Bleeding Following Factor Xa Inhibitor Treatment: Postmarketing Surveillance Study in Japan.

JACC. Asia·2026
Same author

Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouse.

Fluids and barriers of the CNS·2026
Same author

Human Brain Vasculature-on-a-Chip Model Constructed With Microvessels Isolated From Cryopreserved Postmortem Human Brain Tissue.

Advanced healthcare materials·2026
Same author

Hydrogel Embedding of Mesenchymal Stem Cells Supports Extracellular Vesicle Production.

Biotechnology and bioengineering·2026

Related Experiment Video

Updated: Jul 31, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.7K

Generalized Strategy for Engineering Mammalian Cell-Compatible RNA-Based Biosensors from Random Sequence Libraries.

Everett R Allchin1, Jonah C Rosch1, Alexander D Stoneman1

  • 1Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37212, United States.

ACS Sensors
|May 8, 2023
PubMed
Summary

Researchers developed a new method to create fluorescent RNA biosensors for detecting molecules in cells. This approach expands the range of detectable targets, enabling real-time monitoring in mammalian systems.

Keywords:
RNASELEXaptamerbiosensorin vitro selectionmammalian cells

More Related Videos

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

3.8K
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

5.5K

Related Experiment Videos

Last Updated: Jul 31, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.7K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

3.8K
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

5.5K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Cellular Imaging

Background:

  • Fluorescent RNA biosensors enable real-time molecular detection in living cells.
  • Current biosensors rely on riboswitches, limiting the range of detectable targets.
  • Existing riboswitches are known for target specificity and conformational changes upon binding.

Purpose of the Study:

  • To develop a novel framework for creating mammalian cell-compatible RNA biosensors.
  • To overcome the limitation of a restricted number of known riboswitches for biosensor design.
  • To demonstrate a proof-of-concept for detecting custom targets using this new framework.

Main Methods:

  • Utilized a framework for producing mammalian cell-compatible biosensors.
  • Employed aptamers selected from a large random library via Capture-SELEX.
  • Generated and characterized a fluorescent RNA biosensor targeting L-dopa.

Main Results:

  • Successfully generated a fluorescent RNA biosensor against L-dopa, a neurotransmitter precursor.
  • Demonstrated the framework's capability to create biosensors for custom targets.
  • Validated the utility of aptamer selection for biosensor development.

Conclusions:

  • The developed framework enables the creation of RNA biosensors for custom targets in mammalian cells.
  • This approach overcomes limitations associated with traditional riboswitch-based biosensors.
  • The method holds significant promise for advancing real-time molecular detection in biological research.