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Related Experiment Video

Updated: Jul 31, 2025

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping.

Chungha Lee, Herve Hugonnet, Juyeon Park

|May 9, 2023

Summary

This summary is machine-generated.

This study introduces a fast, single-shot refractive index (RI) imaging method for cells and tissues. The technique enables rapid, quantitative RI mapping of biological samples with subcellular resolution.

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Last Updated: Jul 31, 2025

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

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Area of Science:

Biomedical Optics
Quantitative Phase Imaging
Cellular Imaging

Background:

Refractive index (RI) is a vital intrinsic contrast parameter in pathophysiology.
Existing 3D quantitative phase imaging methods for RI measurement are often slow and require complex setups.

Purpose of the Study:

To develop a single-shot refractive index imaging method.
To enable rapid and sensitive RI visualization of biological samples.

Main Methods:

Spectral multiplexing and optical transfer function engineering were employed.
Three color-coded intensity images were acquired simultaneously in a single shot.
Deconvolution of intensity images yielded the RI map of the in-focus slice.

Main Results:

A proof-of-concept setup using Fresnel lenses and a liquid-crystal display was constructed.
The method was validated using microspheres and simulations.
Subcellular resolution RI imaging of static and dynamic biological cells was achieved.

Conclusions:

The proposed method offers a fast and efficient approach for single-shot RI slice imaging.
This technique has potential for noninvasive, quantitative assessment of biological samples.