Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Author Correction: PCM1 orchestrates centrosomal and flagellar protein transport to promote sperm maturation.

Communications biology·2026

Same author

Development of the Surgical Implantation and Fixation of an Implanted Middle Ear Microphone, the "UmboMic," in Cadaveric Sheep.

Journal of the Association for Research in Otolaryngology : JARO·2026

Same author

Multi-site temporal control of optogenetic stimulation enhances firing frequencies in peripheral nerves.

bioRxiv : the preprint server for biology·2026

Same author

Prognostic Significance and Immune Correlation of CCL3 Expression in Colon Adenocarcinoma: Insights From Multidatabase Analysis.

Biochemistry research international·2026

Same author

Fecal Microbiota Transplantation for Gastrointestinal Complications After Allogeneic Hematopoietic Cell Transplantation: A Systematic Review and Narrative Synthesis.

Transplantation and cellular therapy·2026

Same author

Unlocking a Novel Pathway for Rapidly Generating Effective Solid Electrolyte Interface Layer Inspired by tRNA Working Mechanism.

Advanced materials (Deerfield Beach, Fla.)·2026

Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026

Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026

Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026

Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026

Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026

Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 31, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

MicroLED light source for optical sectioning structured illumination microscopy.

Vikrant Kumar, Keith Behrman, Forest Speed

|May 9, 2023

Summary

This summary is machine-generated.

A novel microLED microdisplay enables optical sectioning structured illumination microscopy (OS-SIM) for improved deep tissue imaging. This bright, compact light source enhances contrast in widefield microscopy, offering new possibilities for biological research.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Related Experiment Videos

Last Updated: Jul 31, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Area of Science:

Biophotonics
Microscopy
Optical Engineering

Background:

Optical sectioning structured illumination microscopy (OS-SIM) traditionally requires complex illumination pattern generation.
Existing methods using SLMs, laser interference, or DMDs are often too complex for miniscope systems.
MicroLEDs offer a bright, compact alternative for patterned illumination.

Purpose of the Study:

To develop a directly addressable striped microLED microdisplay for OS-SIM.
To characterize the microLED microdisplay's performance.
To demonstrate OS-SIM's optical sectioning capability in a benchtop setup.

Main Methods:

Designed and fabricated a 100-row striped microLED microdisplay on a flexible cable.
Performed luminance-current-voltage characterization of the microLEDs.
Implemented OS-SIM using the microLED microdisplay to image a fixed mouse brain slice.

Main Results:

The microLED microdisplay was successfully designed and characterized.
OS-SIM imaging of a GFP-labeled mouse brain slice demonstrated optical sectioning.
Reconstructed OS-SIM images showed an 86.92% contrast improvement compared to pseudo-widefield (44.31%).

Conclusions:

MicroLED microdisplays are a viable and effective light source for OS-SIM.
This technology offers enhanced contrast and optical sectioning for deep tissue widefield imaging.
MicroLED-based OS-SIM presents a new capability for advanced biological imaging applications.