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Two-dimensional electrophoretic studies of platelets from dogs affected with basset hound hereditary thrombopathy: a
Because of a thrombasthenia-like platelet aggregation defect, platelets from dogs affected with Basset Hound Hereditary Thrombopathy were compared to normal control dog platelets by three different techniques in order to assess platelet membrane glycoprotein content. Crossed immunoelectrophoresis (CIE), two-dimensional nonreduced-reduced electrophoresis (NR-R), and O'Farrell two-dimensional electrophoresis were used for the assays. CIE and NR-R gels detected no differences between affected Basset Hound and control dog platelets. Gels run by the O'Farrell technique detected no differences in glycoprotein/protein content, however, there appear to be several constituents missing from BHT affected dog platelet samples. The missing components appear to be either lipids or sialoglycoproteins as they were detectable by silver staining but not by Coomassie Blue staining.
Because of a thrombasthenia-like platelet aggregation defect, platelets from dogs affected with Basset Hound Hereditary Thrombopathy were compared to normal control dog platelets by three different techniques in order to assess platelet membrane glycoprotein content. Crossed immunoelectrophoresis (CIE), two-dimensional nonreduced-reduced electrophoresis (NR-R), and O'Farrell two-dimensional electrophoresis were used for the assays. CIE and NR-R gels detected no differences between affected Basset Hound and control dog platelets. Gels run by the O'Farrell technique detected no differences in glycoprotein/protein content, however, there appear to be several constituents missing from BHT affected dog platelet samples. The missing components appear to be either lipids or sialoglycoproteins as they were detectable by silver staining but not by Coomassie Blue staining.