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Effects of storage and 37 degrees C-incubation with fresh plasma on platelet calcium distribution, mobilization,
Aggregability of platelet concentrate decreased progressively during storage at room temperature and recovered when these stored platelets were incubated with fresh plasma at 37 degrees C. During storage, the resting level of cytoplasmic free Ca2+ ([Ca2+]i) which was measured by fluorescence Ca2+ indicator, quin 2, increased. On the other hand, the amount of Ca2+ mobilization and influx after stimulation with ADP or thrombin decreased. After 37 degrees C-incubation of these stored platelets with fresh plasma for 2-3 hours, resting level of [Ca2+]i decreased, and the amount of Ca2+ mobilization and influx increased (recovered). The resting level of [Ca2+]i indicated reverse correlation to aggregability and to the amount of Ca2+ mobilization and influx. These results suggest that the platelet Ca2+ influx in resting or activation state change during storage and by 37 degrees C with fresh plasma. These changes cause the reduction and the restoration of platelet aggregability.
Aggregability of platelet concentrate decreased progressively during storage at room temperature and recovered when these stored platelets were incubated with fresh plasma at 37 degrees C. During storage, the resting level of cytoplasmic free Ca2+ ([Ca2+]i) which was measured by fluorescence Ca2+ indicator, quin 2, increased. On the other hand, the amount of Ca2+ mobilization and influx after stimulation with ADP or thrombin decreased. After 37 degrees C-incubation of these stored platelets with fresh plasma for 2-3 hours, resting level of [Ca2+]i decreased, and the amount of Ca2+ mobilization and influx increased (recovered). The resting level of [Ca2+]i indicated reverse correlation to aggregability and to the amount of Ca2+ mobilization and influx. These results suggest that the platelet Ca2+ influx in resting or activation state change during storage and by 37 degrees C with fresh plasma. These changes cause the reduction and the restoration of platelet aggregability.