Related Experiment Video
Updated: Jul 31, 2025

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Motility related gene expression of Campylobacter jejuni NCTC 11168 derived from high viscous media
Yulan Su1, Thomas Alter1, Greta Gölz1
1Institute of Food Safety and Food Hygiene, Freie Universität Berlin, Berlin, 14163, Germany.
Abstract:
Flagellation is one of the major virulence factors of Campylobacter jejuni (C. jejuni), enabling bacterial cells to swarm in rather high viscous fluids. The aim of this study was to determine the impact of the surrounding viscosity on the expression of motility related genes of C. jejuni. Therefore, bacterial RNA was extracted from liquid cultures as well as from bacterial cells recovered from the edge and the center of a swarming halo from high viscous media. The expression pattern of selected flagellar and chemotaxis related genes was investigated by RT-PCR. Higher mRNA levels of class 1 and lower levels of class 2 and 3 flagellar assembly genes were detected in cells derived from the edge of a swarming halo than in cells from the center. This indicates different growth states at both locations within the swarming halo. Furthermore, higher mRNA levels for energy taxis and motor complex monomer genes were detected in high viscous media compared to liquid culture, indicating higher demand of energy if C. jejuni cells were cultivated in high viscous media. The impact of the surrounding viscosity should be considered in future studies regarding motility related questions.
More Related Videos
07:59Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
Published on: May 10, 2020
05:34Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
Related Concept Videos
Flagella and Motility in Bacteria
Chemotaxis in E. coli
Stringent Response in E. coli
Coordination of Gene Expression Processes in Bacteria
Bacterial Translocation and Protein Secretion
Gram-negative Bacterial Protein Secretion Systems