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High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline
Guanning Wang1, Olga Lyudovyk2, Justin Y Kim3
1Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
STAR Protocols
|May 9, 2023
Summary
This study introduces a high-throughput immune profiling pipeline to analyze how immune responses in patients affect clinical outcomes in immunotherapy trials. This method links immune cell data to patient results for better drug development.
Area of Science:
- Immunology
- Computational Biology
- Clinical Research
Background:
- Immunotherapy clinical trials are rapidly increasing, offering chances to study drug effects on the immune system.
- Understanding the link between immune responses and clinical outcomes is crucial for novel drug development.
Purpose of the Study:
- To present a protocol for high-throughput immune profiling of clinical cohorts.
- To correlate immune responses with clinical outcomes in immunotherapy.
Main Methods:
- Development of the Human Immune Profiling Pipeline.
- End-to-end solution from flow cytometry data to computational analysis.
- Unsupervised patient clustering based on lymphocyte landscape.
Main Results:
- The pipeline enables large-scale immune profiling.
- Facilitates the study of immune responses in clinical cohorts.
- Identifies patient clusters based on immune cell profiles.
Conclusions:
- The Human Immune Profiling Pipeline is a comprehensive tool for dissecting immune mechanisms in clinical trials.
- This protocol aids in understanding how immune profiles impact patient outcomes in immunotherapy.
