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In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing.

Balázs Kakuk1, Ákos Dörmő1, Zsolt Csabai1

  • 1Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720, Szeged, Hungary.

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|May 10, 2023
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Summary

This study presents the first long-read RNA sequencing of the monkeypox virus transcriptome. This research provides a comprehensive view of viral and host gene expression during infection, enhancing our understanding of orthopoxvirus biology.

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Area of Science:

  • Virology
  • Genomics
  • Transcriptomics

Background:

  • The recent human monkeypox outbreak highlights the need for detailed studies on orthopoxviruses.
  • The transcriptome of the monkeypox virus has not been previously characterized using either short- or long-read sequencing.
  • Understanding viral transcriptomes is crucial for characterizing viral biology and host-pathogen interactions.

Purpose of the Study:

  • To generate and analyze the first long-read RNA sequencing dataset of the monkeypox virus transcriptome.
  • To enable in-depth characterization of the monkeypox virus's transcriptomic architecture.
  • To investigate viral and host gene expression changes during infection.

Main Methods:

  • Utilized Oxford Nanopore long-read RNA sequencing.
  • Sequenced both direct cDNA and native RNA.
  • Analyzed viral and host transcriptomic data.

Main Results:

  • Generated a comprehensive dataset for monkeypox virus transcriptome analysis.
  • The data facilitates the characterization of viral transcriptomic architecture.
  • Enabled estimation of gene expression changes in both virus and host cells.

Conclusions:

  • This study fills a critical gap in understanding the monkeypox virus transcriptome.
  • The generated dataset is a valuable resource for future research on orthopoxviruses.
  • Provides a deeper understanding of viral infection-induced alterations at the transcriptome level.