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Related Experiment Videos

Interaction of pneumococcal antigens with complement in rats.

J D Coonrod, S Jenkins

    Infection and Immunity
    |March 1, 1979
    PubMed
    Summary

    Pneumococcal capsular polysaccharides weakly activate complement (C3-C9). Other pneumococcal antigens, not polysaccharides, may cause hypocomplementemia during infection.

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    Area of Science:

    • Immunology
    • Microbiology

    Background:

    • Streptococcus pneumoniae infections can lead to hypocomplementemia.
    • The role of pneumococcal components, particularly capsular polysaccharides, in complement activation is not fully understood.

    Purpose of the Study:

    • To investigate the in vitro and in vivo complement activation by pneumococcal antigens.
    • To determine the contribution of capsular polysaccharides to complement consumption during pneumococcal infection.

    Main Methods:

    • In vitro complement activation assays using rat serum and whole pneumococci or purified capsular polysaccharides.
    • In vivo studies involving intravenous injection of pneumococci or capsular polysaccharides into rats.
    • Serum complement component C3-C9 levels were measured.
    • Immunofluorescence and histological examination of tissues.

    Main Results:

    • Whole pneumococci activated complement (C3-C9) in rat serum, with variations among serotypes.
    • Purified capsular polysaccharides activated complement only at high concentrations and were less effective than whole organisms.
    • Intravenous injection of whole pneumococci caused a transient decline in serum C3-C9, while capsular polysaccharides did not.
    • Circulating capsular polysaccharides deposited in tissues without detectable C3 or inflammatory response.

    Conclusions:

    • Pneumococcal capsular polysaccharides are weak activators of complement.
    • Other pneumococcal antigens are likely more significant contributors to hypocomplementemia in pneumococcal infections.

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