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Related Experiment Video

Updated: Jul 31, 2025

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Current trends in RNA virus detection through metatranscriptome sequencing data.

So Nakagawa^1,2,3,4, Shoichi Sakaguchi⁵, Atsushi Ogura⁶

¹Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.

|May 10, 2023

Summary

Metatranscriptome sequencing reveals numerous novel RNA viruses in diverse environments and hosts. This review highlights key studies, bioinformatics tools for RNA virus discovery, and links to host genomes.

Keywords:

RNA virome RNA-dependent RNA polymerase SARS-CoV-2 endogenous viral elements metatranscriptome

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Area of Science:

Virology
Genomics
Bioinformatics

Background:

Advances in sequencing technology enable large-scale RNA virus discovery.
Metatranscriptomics has uncovered a vast diversity of previously unknown RNA viruses.
RNA viruses play significant roles in various ecosystems and host health.

Purpose of the Study:

To review recent RNA virome studies from diverse sources.
To focus on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and related virus identification.
To introduce bioinformatics tools for RNA virus discovery.

Main Methods:

Metatranscriptome sequencing of environmental and biological samples.
Bioinformatic analysis for RNA virus identification.
Comparative genomics to explore host-virus relationships.

Main Results:

Numerous novel RNA viruses have been identified across invertebrate, vertebrate, and aquatic environments.
Metatranscriptomics is a powerful tool for discovering diverse RNA viruses, including SARS-CoV-2.
New bioinformatics software and databases facilitate RNA virus identification.

Conclusions:

Metatranscriptomics has revolutionized RNA virus discovery.
Understanding the RNA virome is crucial for public health and ecological studies.
Further research is needed to explore the roles of newly identified RNA viruses and their interactions with host genomes.