Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

809
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
809
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

662
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
662
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

835
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
835
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

756
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
756
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Resolution Ion Mobility-Based Isotopic Shifts of Dimethylated Peptide Isomers.

Analytical chemistry·2026
Same author

Spatial Ion Compression without Loss of Resolution on a Cyclic Ion Mobility Spectrometry-Mass Spectrometry Platform.

Analytical chemistry·2025
Same author

Improving Peak Capacity in Glycan Ion Mobility Separations through Traveling Wave-Based Ion Heating.

Journal of the American Society for Mass Spectrometry·2025
Same author

Integrating High-Resolution Cyclic Ion Mobility Separations with Tandem Mass Spectrometry and Collision Cross Section Measurements for Human Milk Oligosaccharide Sequencing.

ACS measurement science au·2025
Same author

Developing Reduced Mass-Only Ion Mobility Separations to Unravel Mass Distribution-Based Isotopic Shifts.

Journal of the American Society for Mass Spectrometry·2025
Same author

Determining β-Monosaccharide Head Group Composition with High-Resolution Cyclic Ion Mobility Separations Coupled to Tandem Mass Spectrometry as a First Step for Unknown Cerebroside Analysis.

International journal of mass spectrometry·2025

Related Experiment Video

Updated: Jul 31, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K

General Method to Obtain Collision Cross-Section Values in Multipass High-Resolution Cyclic Ion Mobility Separations.

Sanaz C Habibi1, Gabe Nagy1

  • 1Department of Chemistry, University of Utah, 315 South 1400 East, Room 2020, Salt Lake City, Utah 84112, United States.

Analytical Chemistry
|May 10, 2023
PubMed
Summary

A new method uses average ion velocities to calculate collision cross sections (CCS) in cyclic ion mobility-mass spectrometry (cIMS-MS), enabling quantitative biomolecule characterization. This robust approach accurately determines CCS for complex molecules like glycans, even with anomer splitting.

More Related Videos

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

24.7K
Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
08:51

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

10.4K

Related Experiment Videos

Last Updated: Jul 31, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

24.7K
Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
08:51

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

10.4K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectrometry

Background:

  • Ion mobility spectrometry-mass spectrometry (IMS-MS) is crucial for omics research, but separations are often qualitative.
  • Collision cross section (CCS) is a key quantitative metric in IMS-MS for biomolecule characterization.
  • Calculating CCS in multipass IMS systems like cyclic IMS-MS (cIMS-MS) is challenging due to method limitations and calibrant requirements.

Purpose of the Study:

  • To develop a general method for calculating CCS values in cIMS-MS-based separations using average ion velocities.
  • To address the challenges of quantitative CCS determination in multipass IMS systems.
  • To enable accurate characterization of biomolecules, including glycans with potential anomer splitting.

Main Methods:

  • Developed calibration curves using common CCS calibrants (tetra-alkylammonium salts, polyalanine, hexakis(fluoroalkoxy)phosphazines) under various traveling wave (TW) conditions.
  • Calculated cIMS CCS values and compared them with established drift tube IMS CCS measurements.
  • Applied the method to analyze glycan species (2α-mannobiose and melibiose) to observe and assign unique CCS values for anomers.

Main Results:

  • Calculated cIMS CCS values showed less than ~1% error compared to drift tube IMS CCS measurements.
  • Observed anomer splitting for 2α-mannobiose and melibiose, assigning two unique CCS values for each glycan.
  • Demonstrated the robustness and accuracy of the average ion velocity method for CCS determination in multipass IMS.

Conclusions:

  • The average ion velocity method provides a robust approach for obtaining accurate CCS values in cIMS-MS.
  • This methodology successfully characterized glycan anomers, a first for this type of analysis.
  • The method is anticipated to be applicable to various IMS-MS platforms with multipass separations and aid in future CCS database development.