Tb³⁺-Based Off-On Fluorescent Platform for Multicolor and Dosage-Sensitive Visualization of Bacterial Spore Marker

Developing a novel strategy for the sensitive and rapid detection of pathogenic bacterial spores in field or on-site settings will be helpful in minimizing their potential threats to human health, environmental safety, and food safety. In this study, Tb³⁺ was combined with glutathione (GSH)-modified copper nanoclusters (CuNCs), and an aggregation-induced emission (AIE) fluorescent probe based on Tb-GSH-CuNCs was fabricated for dipicolinic acid (DPA, a pathogenic bacterial spore marker) sensing. Making use of the competitive binding of Tb³⁺ between GSH-CuNCs and DPA, a multicolor sensing of DPA was facilely realized without introducing fluorescent materials as the reference. Due to an "off-on" response mechanism of the AIE fluorescent probe, this multicolor response to DPA exhibited a feature of rich color gradients and highly discriminative color change, allowing a dosage-sensitive visual quantification of DPA. The DPA with a concentration even as low as 0.5 μM can still be identified by the naked eye. Moreover, together with a smartphone app, which can extract the R (red), G (green), and B (blue) values from the probe system, a portable platform can be established for sensitive DPA quantification in the range of 0.5-70 μM, showing great potential for the practical monitoring of DPA in field or on-site settings.