Silicon dioxide nanoparticles compromise decidualization via autophagy impairment to possibly cause embryo resorption

The wide application of silicon dioxide nanoparticles (SiO₂NPs) has raised concerns about their harmful effects on reproduction. The purpose of this research was to investigate the toxic effects and the possible mechanisms by which SiO₂NPs affect decidualization and pregnancy progression. We found that SiO₂NPs could inhibit decidualization, both in mice and in human endometrial stromal cells (HESCs). Embryo resorption was also evident in mice treated with SiO₂NPs. When HESCs were treated with SiO₂NPs, decidualization was inhibited and there was an increase in intracellular lysosomes and autophagosomes as well as the blockage of autophagic flux. Interestingly, a reduction of autophagosome accumulation via 3-methyladenine (3MA) significantly restored the decidualization of HESCs. In summary, our results indicate that SiO₂NPs can affect embryo survival by impairing decidualization through a dysfunctional autophagic process.