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Updated: Jul 30, 2025

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Hyperspectral imaging in forensic science: An overview of major application areas
Kristiane de Cássia Mariotti1, Rafael Scorsatto Ortiz2, Marco Flôres Ferrão3
1Núcleo de Identificação, Polícia Federal, Rio Grande do Sul, Brazil; Instituto Nacional de Ciência e Tecnologia Forense - INCT FORENSE, Rio Grande do Sul, Brazil; Instituto de Química, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil.
Abstract:
Analysis of evidence is a challenge. Crime scene materials are complex, diverse, sometimes of an unknown nature. Forensic science provides the most critical applications for their examination. Chemical tests, analytical methods, and techniques to process the evidence must be carefully selected by the forensic scientist. Ideally, it may be interpreted, analyzed, and judged in the original context of the crime scene. In this sense, hyperspectral imaging (HSI) has been employed as an analytical tool that maintains the integrity of the samples/objects for multiple and sequential analysis and for counter-proof exams. This paper is an overview of forensic science trends for the application of HSI techniques in the last ten years (2011-2021). The examination of documents was the main area of exploration, followed by bloodstain analysis aging process; trace analysis of explosives and gunshot residue. Chemometric tools were also addressed since they are crucial to obtain the most important information from the samples. There are great challenges in applying HSI in forensic science, but there have been clear technological and scientific advances, and a solid foundation has been built for the use of HSI in real-life cases.
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