Related Experiment Video
Updated: Jul 30, 2025
11:47
Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
1.7K
Influence of Disinfection Methods on Cinematographic Film
Vítězslav Knotek1, Michal Ďurovič1, Bohumil Dolenský2
1Department of Chemical Technology of Monument Conservation, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Materials (Basel, Switzerland)
|May 13, 2023
Summary
This study evaluated disinfectants for cellulose triacetate films, finding butanol vapors and Septonex® to be gentlest. Other disinfectants like alcohol mixtures and ethylene oxide caused plasticizer loss or reduced viscosity in film samples.
Area of Science:
- Film preservation
- Microbiology
- Material science
Background:
- Microbial contamination poses a significant threat to cinematographic films, potentially causing irreversible image data loss.
- Cellulose triacetate, a common film base since the 1940s, exhibits resistance to solvents but can degrade due to microbial activity.
- Effective disinfection methods are crucial for preserving historical film archives.
Purpose of the Study:
- To assess the impact of four mass-disinfection agents on the properties of cellulose triacetate film.
- To identify disinfectants that minimize damage to the film base while remaining effective against microorganisms.
- To evaluate changes in optical, mechanical, and chemical properties after disinfection and accelerated aging.
Main Methods:
- Four disinfectants were tested: butanol vapors, Bacillol® AF (alcohol mixture), Septonex® (quaternary ammonium bromide solution), and ethylene oxide gas with carbon dioxide.
- Cellulose triacetate film samples underwent disinfection followed by aging for 56 days at 70°C and 55% relative humidity.
- Optical, mechanical, and chemical properties, including degree of substitution and intrinsic viscosity, were analyzed.
Main Results:
- No tested disinfectant altered the degree of substitution of cellulose triacetate.
- Bacillol® AF extraction of plasticizer (triphenyl phosphate) and reduced intrinsic viscosity were observed after aging.
- Ethylene oxide disinfection also resulted in a slight decrease in intrinsic viscosity.
- Butanol vapors and Septonex® showed the least impact on film properties compared to controls.
Conclusions:
- Butanol vapors and Septonex® are recommended as the most suitable disinfectants for cellulose triacetate film bases under the tested conditions.
- Careful selection of disinfection agents is vital to prevent material degradation and preserve film integrity.
- Further research may explore long-term effects and efficacy against a broader spectrum of microbes.
More Related Videos
10:17Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
Published on: October 9, 2016
15.4K
13:38Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
Published on: May 10, 2013
30.7K
Related Concept Videos
01:26Physical Methods for Controlling Microbial Growth: Radiation and Filtration
144
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
144
01:30Cleaning, Sterilization, and Disinfection
7.2K
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
7.2K
01:30Methods of Sterilization II: Chemical Methods
6.7K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
6.7K
01:29Methods of Sterilization I: Physical Methods
20.3K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
20.3K
01:28Antimicrobial Effectiveness
99
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
99
01:27Chemical Agents for Microbial Control
142
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
142